WebIn the laboratory, the most common induction method is the DNA damaging chemical Mitomycin C. Although pH has been listed in the literature as an induction method, it is not widely used. Here, we detail a protocol for prophage induction by … WebDNA synthesis is selectively inhibited in Salmonella typhimurium by treatment with the antibiotic mitomycin C. These inhibited cells will, however, produce active particles on infection with phage P22. Genetic recombination between marked phages is not affected, but the frequency of lysogenization is drastically reduced under these conditions.

Mitomycin C: mechanism of action, usefulness and limitations

Web24 sep. 2024 · Genome analysis of representative strains of the 7 genetic lineages of Lactococcus lactis constituting the culture indicated the presence of prophages in … WebMitomycin C has also been used topically rather than intravenously in several areas. The first is cancers, particularly bladder cancers and intraperitoneal tumours. It is now well known that a single instillation of this agent within 6 hours of bladder tumor resection can prevent recurrence. frontenac park reservations

National Center for Biotechnology Information

WebInduction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. … WebMitomycin C (215a) shows a potent activity against a wide spectrum of tumors and bacteria. In spite of its high toxicity it is extensively used in chemotherapy especially in treating gastrointestinal cancers 〈B-80MI 801-01〉. For synthetic chemists the structure of mitomycins presents a formidable challenge. WebProphage Induction with mitomycin C (1ug/ml) gave me reduction in bacterial turbidity measured at OD 600 nm. A spot test of the lysate had given a noticeable clearance. ghost fn