Ire bp
WebElution of IRE-BP was accom- plished with 1M KCI, 5 mg of sodium heparin per milliliter, 25 mM tris-CI, pH 8, and 1% Triton X- 100. Based on recovery of IRE-binding activity and the recovery of labeled K562 protein from a parallel experiment with a [35S]methionine lysate, we esti- mate that the IRE-binding protein was purified WebDec 15, 1992 · IRE-BP produced in iron-replete cells has aconitase (EC 4.2.1.3) activity. The protein shows extensive sequence homology with mitochondrial aconitase, and sequences of peptides prepared from cytosolic aconitase are identical with peptides of IRE-BP. As an active aconitase, IRE-BP is expected to have an Fe-S cluster, in analogy to other ...
Ire bp
Did you know?
Webing of the IRE-BP to IREs is reversibly regulated by the iron status of the cell. The IRE-BP is highly conserved among human, rat, mouse, and rabbit, and it is identical to the cytosolic form of aconitase. In this study, we dem- onstrate that a distinct human gene encoding a protein which is 57% identical to the initially described IRE-BP, WebThe iron-responsive element binding protein (IRE- BP) is a cytosolic protein that binds a highly conserved sequence in the untranslated regions of mRNAs in- volved in iron metabolism including ferritin, transfer- rin receptor, and erythroid 6-aminolevulinate acid synthase. This conserved sequence is termed the iron-
WebThe iron-responsive element-binding protein (IRE-BP) binds to specific RNA stem-loop structures called iron-responsive elements (IREs), which mediate the post-transcriptional regulation of a variety of genes involved in iron metabolism. The IRE-BP is cytosolic aconitase, and a [4Fe-4S] cubane cluster is required for aconitase activity but is … WebThis protein is referred to by different groups as “iron regulatory factor (IRF)”, “IRE-binding protein (IRE-BP)”, “ferritin repressor protein (FRP)” or “p90”. Keywords. Translational …
http://iehp.org/ WebUpdate your information, check eligibility, print your temporary IEHP Card, view medicine history, change your doctor, and more. Member Login.
WebMar 17, 1992 · The iron regulatory element (IRE) in the 5'-untranslated region of ferritin mRNA interacts with a specific regulator protein (P-90, IRE-BP, or FRP) to block translation. High cellular iron changes the IRE/P-90 interaction to relax the translational block and allow polyribosome formation.
WebNov 16, 2005 · IREs are regulatory elements that encode a phylogenetically defined sequence-structure motif that provide a specific recognization site for the IRE-binding proteins (IRE-BP1 or IRE-BP2). Iron starvation of cells induces high affinity binding of cytoplasmic IRE-BP to an IRE, which has at least two distinct known biological … chunky baseboardsWeb(15) Binding of the IRE-BP represses ferritin translation and represses degradation of the TfR mRNA. (16) Yet in doing so it presents an angled mirror-polished cliff face of glass, which has been reflecting the sun straight across into the Lloyds offices across the road – the seminal "inside-out" machine that Rogers designed 30 years earlier ... detention officer mecklenburg countyWebJan 18, 1994 · The iron-responsive element-binding protein (IRE-BP) binds to specific stem-loop RNA structures known as iron-responsive elements (IREs) present in a variety of cellular mRNAs (e.g., those encoding ferritin, erythroid 5-aminolevulinate synthase, and transferrin receptor). Expression of these genes is regulated by interaction with the IRE-BP. chunky bar whiteWebThe iron-responsive element-binding protein (IRE-BP) is a cytosolic RNA-binding protein that functions in the maintenance of iron homeostasis by post-transcriptionally regulating … chunky base bootsWebWhich of the following would ONLY be found in a cell in which the RNA binding domain of IRE-BP has been mutated so that it can no longer bind IRES? ferritin protein in low iron transferrin mRNA in low iron ferritin protein in high iron transferrin mRNA in high iron . Previous question Next question. COMPANY. detention officer pay ukWebThe iron-responsive element binding protein (IRE-BP) is a cytosolic protein that binds a highly conserved sequence in the untranslated regions of mRNAs involved in iron … chunky bar recipeWebDec 15, 1992 · IRE-BP is required in the posttranscriptional regulation of ferritin mRNA translation and stabilization of transferrin receptor mRNA. In spite of substantial homology between the amino acid sequences of mammalian mitochondrial aconitase and IRE-BP, the mitochondrial protein does not bind IREs. chunky bars where to buy