WebWe examined the toxic effects of glyphosate on diploid (DIMF) and triploid (TRMF) fin cell lines from the Oriental Weather Loach Misgurnus anguillicaudatus. The LC 50 values of glyphosate estimated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay were 315.34 and 371.77 mg/L for DIMF and TRMF, respectively. Superoxide dismutase (SOD ... WebAug 24, 2024 · H295R is a human adreno-carcinoma cell line that expresses the genes encoding these key steroidogenesis enzymes. This means the H295R cell system …
The OECD validation program of the H295R steroidogenesis assay: …
WebApr 18, 2011 · A high-throughput assay using H295R human adrenocortical carcinoma cells was used to evaluate the effect of 2,060 chemical samples on steroidogenesis via HPLC-MS/MS quantification of 10 steroid ... WebContract Laboratory needed for Steroidogenesis H295R assay testing. I need some info about the assay and how you run it, such as which steroids you can evaluate, the detection method you use and the sensitivity of this method. Could we organize a meeting together to discuss about those points and the other questions I have? black calf leather
Endocrine Disruptor Screening Program Tier 1 Battery of Assays
WebTable 6.2: Core chemicals and their hypothesized mode of action selected for the H295R Steroidogenesis Assay validation studies. Due to the nature of the validation studies, … Websteroidogenesis, specifically the production of 17β-estradiol (E2) and testosterone (T) was carried out in a multi–step process. The H295R assay has been optimized and validated (2)(3)(4)(5). 2. The objective of the H295R Steroidogenesis Assay is to detect substances that affect production of E2 and T. WebJan 1, 2024 · exposure of steroidogenic active H295R cells to HT042 is non-cytotoxic to various concentrations 100, 200, and 500 mg /mL and found HT042 have not shown the changes in H295R steroidogenesis... black calf names