WebSep 2, 2024 · A FlipGFP reporter assay was performed to screen for potential inhibition of 3CLpro by the identified drugs at a single concentration (10µM). Shown are the drugs that showed a statistically ... WebGeneral Tools: Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that may be useful for detection assays and more. Research Articles: A list of free access articles on COVID-19 research. Protocols: Protocols that may be useful for COVID-19 research. ... FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro. (Unpublished)

Figure 4. Development of cell-based FlipGFP assay for the...

FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence after cleavage by executioner caspases. Failure for cells to die or cells dying at the wrong time and/or place can lead to an array of diseases and … See more GFP contains 11 β-strands and one central ɑ-helix that can be split into 3 parts: (1) β-strands 1-9 (β1-9) and the one ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and … See more To add to the fluorogenic reporter toolkit, the Shu Lab also designed a red fluorogenic protease reporter based on superfolder Cherry … See more The Shu Lab successfully used FlipGFP to study apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells expressing FlipGFP with staurosporine, which … See more northamericangamebird.com

Discovery of SARS-CoV-2 Papain-like Protease Inhibitors through a ...

WebJan 1, 2024 · target engagement of our rationally designed Mproinhibitors. The FlipGFP assay was also applied to validate the structurally disparate Mproinhibitors reported in the literature. Lastly, we introduce recent progress in identifying naturally occurring Mpromutants that are resistant to nirmatrelvir from genome WebSignificantly, we developed a cell-based FlipGFP assay that can be applied to predict the cellular antiviral activity of PLpro inhibitors in the BSL-2 setting. X-ray crystal structure of PLpro in complex with GRL0617 showed that binding of GRL0617 to SARS-CoV-2 induced a conformational change in the BL2 loop to a more closed conformation. WebApr 13, 2024 · Next, we extended the study of reporter assay (Supplementary Fig. 1) ... (Supplementary Fig. 2), in which an inactive flipGFP could be activated by a specific cleavage of NSP5 recognition site. how to repair auto vinyl roof